Coding
Part:BBa_K2997003
Designed by: Ting-Han Sung Group: iGEM19_NCKU_Tainan (2019-10-12)
Sigma 54-dependent transcriptional regulator (pchR)
Background
The pchR gene is responsible for the production of activator proteins that can bind with the inducer (p-Cresol) and leads to the conformation of the protein. [1][2] In other words, the activator protein can bind with p-Cresol to form a protein-p-Cresol complex that will then bind onto the p-Cresol sensing region located just downstream of the pchR gene. The binding of the protein-p-Cresol complex initiates the expression of the downstream gene, in our case GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1738
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1151
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1196
Illegal AgeI site found at 179
Illegal AgeI site found at 1079 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1105
References
- Cho, Ah Ra, Eun Jin Lim, Yaligara Veeranagouda, and Kyoung Lee* Department of Microbiology, Changwon National University, Changwon-si, Kyongnam 641-773, Korea . Identification of a p-Cresol Degradation Pathway by a GFP-Based Transposon in Pseudomonas and Its Dominant Expression in Colonies
- Merike Jo ˜esaar, Eeva Heinaru, Signe Viggor, Eve Vedler & Ain Heinaru Institute of Molecular and Cell Biology, Tartu University, Tartu, Estonia. Diversity of the transcriptional regulation of the pch gene cluster in two indigenous p-Cresol-degradative strains of Pseudomonas fluorescens
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